2019-02-19 17:28:492025-05-28 06:51:35
locus
BSU22480
BSU_22480
outlinks
bsu
BSU22480
BSU_22480
The protein
Catalyzed reaction/ biological activity
Glycerone phosphate = methylglyoxal + phosphate (according to Swiss-Prot)
dihydroxyacetone phosphate --> methylglyoxal + phosphate (according to UniProt)
Biological materials
Mutant
MGNA-A440 ([[gene|mgsA]]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/440 NBRP B. subtilis, Japan]
GP67 ([[gene|mgsA]]::''tet'') [Pubmed|21992469], available in [SW|Jrg Stlke]'s lab
GP84 (''mgsA''::''pX2''(''cat'')), available in [SW|Jrg Stlke]'s lab
BKE22480 ([[gene|mgsA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTTTCATTGTTTATCCCC, downstream forward: _UP4_GACCTTCTTCGGGGAGAAGA
BKK22480 ([[gene|mgsA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTTTCATTGTTTATCCCC, downstream forward: _UP4_GACCTTCTTCGGGGAGAAGA
MGNA-A440 ([[gene|mgsA]]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/440 NBRP B. subtilis, Japan]
GP67 ([[gene|mgsA]]::''tet'') [Pubmed|21992469], available in [SW|Jörg Stülke]'s lab
GP84 (''mgsA''::''pX2''(''cat'')), available in [SW|Jörg Stülke]'s lab
BKE22480 ([[gene|mgsA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTTTCATTGTTTATCCCC, downstream forward: _UP4_GACCTTCTTCGGGGAGAAGA
BKK22480 ([[gene|mgsA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTTTCATTGTTTATCCCC, downstream forward: _UP4_GACCTTCTTCGGGGAGAAGA
Biological materials
Expression vectors
pGP1301 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jrg Stlke]'s lab
pGP1180 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jrg Stlke]'s lab
pGP1181 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Jrg Stlke]'s lab
pGP2207 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP1459]), available in [SW|Jrg Stlke]'s lab
pGP2505 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jrg Stlke]'s lab
pGP1301 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
pGP1180 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab
pGP1181 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab
pGP2207 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP1459]), available in [SW|Jörg Stülke]'s lab
pGP2505 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
Biological materials
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jrg Stlke]'s lab [Pubmed|21992469]
B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab [Pubmed|21992469]
Biological materials
FLAG-tag construct
GP86 (spc, based on [SW|pGP1331]) [Pubmed|21992469], available in [SW|Jrg Stlke]'s lab
GP86 (spc, based on [SW|pGP1331]) [Pubmed|21992469], available in [SW|Jörg Stülke]'s lab
labs
[SW|Boris Grke], University of Vienna, Austria
[SW|Jrg Stlke], University of Gttingen, Germany, [http://genmibio.uni-goettingen.de/index.php?id=90 Homepage]
[SW|Boris Görke], University of Vienna, Austria
[SW|Jörg Stülke], University of Göttingen, Germany, [http://genmibio.uni-goettingen.de/index.php?id=90 Homepage]
The protein
Protein family
methylglyoxal synthase family (single member, according to UniProt)
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
MGS-like domain (aa 1-137) (according to UniProt)