BSU22480

BSU_22480

BSU22480

BSU_22480

Glycerone phosphate = methylglyoxal + phosphate (according to Swiss-Prot)

dihydroxyacetone phosphate --> methylglyoxal + phosphate (according to UniProt)

MGNA-A440 ([[gene|mgsA]]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/440 NBRP B. subtilis, Japan]

GP67 ([[gene|mgsA]]::''tet'') [Pubmed|21992469], available in [SW|Jrg Stlke]'s lab

GP84 (''mgsA''::''pX2''(''cat'')), available in [SW|Jrg Stlke]'s lab

BKE22480 ([[gene|mgsA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTTTCATTGTTTATCCCC, downstream forward: _UP4_GACCTTCTTCGGGGAGAAGA

BKK22480 ([[gene|mgsA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTTTCATTGTTTATCCCC, downstream forward: _UP4_GACCTTCTTCGGGGAGAAGA

MGNA-A440 ([[gene|mgsA]]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/440 NBRP B. subtilis, Japan]

GP67 ([[gene|mgsA]]::''tet'') [Pubmed|21992469], available in [SW|Jörg Stülke]'s lab

GP84 (''mgsA''::''pX2''(''cat'')), available in [SW|Jörg Stülke]'s lab

BKE22480 ([[gene|mgsA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTTTCATTGTTTATCCCC, downstream forward: _UP4_GACCTTCTTCGGGGAGAAGA

BKK22480 ([[gene|mgsA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTTTCATTGTTTATCCCC, downstream forward: _UP4_GACCTTCTTCGGGGAGAAGA

pGP1301 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jrg Stlke]'s lab

pGP1180 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jrg Stlke]'s lab

pGP1181 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Jrg Stlke]'s lab

pGP2207 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP1459]), available in [SW|Jrg Stlke]'s lab

pGP2505 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jrg Stlke]'s lab

pGP1301 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

pGP1180 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab

pGP1181 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab

pGP2207 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP1459]), available in [SW|Jörg Stülke]'s lab

pGP2505 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jrg Stlke]'s lab [Pubmed|21992469]

B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab [Pubmed|21992469]

GP86 (spc, based on [SW|pGP1331]) [Pubmed|21992469], available in [SW|Jrg Stlke]'s lab

GP86 (spc, based on [SW|pGP1331]) [Pubmed|21992469], available in [SW|Jörg Stülke]'s lab

[SW|Boris Grke], University of Vienna, Austria

[SW|Jrg Stlke], University of Gttingen, Germany, [http://genmibio.uni-goettingen.de/index.php?id=90 Homepage]

[SW|Boris Görke], University of Vienna, Austria

[SW|Jörg Stülke], University of Göttingen, Germany, [http://genmibio.uni-goettingen.de/index.php?id=90 Homepage]

methylglyoxal synthase family (single member, according to UniProt)

[[this]]

MGS-like domain (aa 1-137) (according to UniProt)